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visium spatial tissue optimization slide reagent kit  (10X Genomics)

 
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    10X Genomics visium spatial tissue optimization slide reagent kit
    Visium Spatial Tissue Optimization Slide Reagent Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visium+spatial+tissue+optimization+slide+reagent+kit/expression+gene+slides+spatial+visium/pmc13176823-41-1-9
    Average 86 stars, based on 1 article reviews
    visium spatial tissue optimization slide reagent kit - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Extraction:

    Article Title: Single cell and spatial transcriptomics highlight the interaction of club-like cells with immunosuppressive myeloid cells in prostate cancer.
    Article Snippet: .. Themost effective extraction time had been established earlier using the Visium Spatial Tissue Optimization Slide & Reagent kit (10X Genomics). ..

    Article Title: Single cell and spatial transcriptomics highlight the interaction of club-like cells with immunosuppressive myeloid cells in prostate cancer
    Article Snippet: .. The most effective extraction time had been established earlier using the Visium Spatial Tissue Optimization Slide & Reagent kit (10X Genomics). ..

    Imaging:

    Article Title: Axonal Eif5a hypusination controls local translation and mitigates defects in FUS-ALS.
    Article Snippet: Imaging was performed on a Nikon-Marzhauser Slide Express 2 whole-slide scanner at ×10 magnification. .. After imaging, sections were permeabilized at 37 °C for 18 min. Permeabilization time was determined using the Visium Spatial Tissue Optimization Slide & Reagent Kit (10x Genomics, PN-1000193) following the Visium Spatial Tissue Optimization User Guide_RevA (10x Genomics, CG000238). ..

    Incubation:

    Article Title: Spatial integration of multi-omics data from serial sections using the novel Multi-Omics Imaging Integration Toolset
    Article Snippet: .. To capture mRNA, tissue sections were incubated with permeabilization enzyme for 12 minutes, which previously had been optimized using the Visium Spatial Tissue Optimization Slide & Reagent kit (10x Genomics, product code 1,000,193). ..



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    Visium Spatial Tissue Optimization Slide Reagent Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visium+spatial+tissue+optimization+slide+reagent+kit/expression+gene+slides+spatial+visium/pmc13176823-41-1-9
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    Spatial RNAseq further suggests boundary identity, cytokinin signaling, and brassinosteroid signaling are increased in drmy1 . A) Cartoon of a typical transverse section of an ap1cal 35S::AP1-GR cauliflower-like cluster, showing the developing flower buds with initiating sepals on the outer ring . B) Representative brightfield images of transverse sections of WT and drmy1 in the ap1cal 35S::AP1-GR background. n=9 Total WT replicate cauliflower-like clusters, n=22 drmy1 replicate cauliflower-like clusters (See also Figure S3). C) An overlay of which single cell cluster maps with the highest prediction score to each <t>Visium</t> spot on the same tissue shown in panel (B). See figure S3 for a more detailed view of prediction scores from each single cell cluster. D) The proportions of Visium spots grouped by which single cell cluster mapped to each with the highest prediction score (clusters that mapped to <10 spots in either genotype were omitted). For cluster 2 (boundaries), WT = 0.102, drmy1 = 0.232. E-G) Overlays of the percentage of transcripts from cytokinin downregulated genes (E), brassinosteroid downregulated genes (F), and SCT normalized expression of DWF1 , a brassinosteroid biosynthesis gene (G). H) Dotplot showing the differences in expression of genes up and down-regulated by various hormones. For each cell, the percentage of transcripts that come from the set of genes up or downregulated by applications of these hormones according to is calculated, then the mean of all the cells in each cluster is calculated for WT and drmy1 . The size of each dot represents the log of the inverse of the p-value between the mean of the two genotypes with a Bonferroni adjusted threshold of p < 0.00069. The color of each dot represents the percentage increase or decrease of the mean of drmy1 compared to WT. Scale bars 500 μm See also: Figure S3, S4
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    Spatial RNAseq further suggests boundary identity, cytokinin signaling, and brassinosteroid signaling are increased in drmy1 . A) Cartoon of a typical transverse section of an ap1cal 35S::AP1-GR cauliflower-like cluster, showing the developing flower buds with initiating sepals on the outer ring . B) Representative brightfield images of transverse sections of WT and drmy1 in the ap1cal 35S::AP1-GR background. n=9 Total WT replicate cauliflower-like clusters, n=22 drmy1 replicate cauliflower-like clusters (See also Figure S3). C) An overlay of which single cell cluster maps with the highest prediction score to each <t>Visium</t> spot on the same tissue shown in panel (B). See figure S3 for a more detailed view of prediction scores from each single cell cluster. D) The proportions of Visium spots grouped by which single cell cluster mapped to each with the highest prediction score (clusters that mapped to <10 spots in either genotype were omitted). For cluster 2 (boundaries), WT = 0.102, drmy1 = 0.232. E-G) Overlays of the percentage of transcripts from cytokinin downregulated genes (E), brassinosteroid downregulated genes (F), and SCT normalized expression of DWF1 , a brassinosteroid biosynthesis gene (G). H) Dotplot showing the differences in expression of genes up and down-regulated by various hormones. For each cell, the percentage of transcripts that come from the set of genes up or downregulated by applications of these hormones according to is calculated, then the mean of all the cells in each cluster is calculated for WT and drmy1 . The size of each dot represents the log of the inverse of the p-value between the mean of the two genotypes with a Bonferroni adjusted threshold of p < 0.00069. The color of each dot represents the percentage increase or decrease of the mean of drmy1 compared to WT. Scale bars 500 μm See also: Figure S3, S4
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    Spatial RNAseq further suggests boundary identity, cytokinin signaling, and brassinosteroid signaling are increased in drmy1 . A) Cartoon of a typical transverse section of an ap1cal 35S::AP1-GR cauliflower-like cluster, showing the developing flower buds with initiating sepals on the outer ring . B) Representative brightfield images of transverse sections of WT and drmy1 in the ap1cal 35S::AP1-GR background. n=9 Total WT replicate cauliflower-like clusters, n=22 drmy1 replicate cauliflower-like clusters (See also Figure S3). C) An overlay of which single cell cluster maps with the highest prediction score to each <t>Visium</t> spot on the same tissue shown in panel (B). See figure S3 for a more detailed view of prediction scores from each single cell cluster. D) The proportions of Visium spots grouped by which single cell cluster mapped to each with the highest prediction score (clusters that mapped to <10 spots in either genotype were omitted). For cluster 2 (boundaries), WT = 0.102, drmy1 = 0.232. E-G) Overlays of the percentage of transcripts from cytokinin downregulated genes (E), brassinosteroid downregulated genes (F), and SCT normalized expression of DWF1 , a brassinosteroid biosynthesis gene (G). H) Dotplot showing the differences in expression of genes up and down-regulated by various hormones. For each cell, the percentage of transcripts that come from the set of genes up or downregulated by applications of these hormones according to is calculated, then the mean of all the cells in each cluster is calculated for WT and drmy1 . The size of each dot represents the log of the inverse of the p-value between the mean of the two genotypes with a Bonferroni adjusted threshold of p < 0.00069. The color of each dot represents the percentage increase or decrease of the mean of drmy1 compared to WT. Scale bars 500 μm See also: Figure S3, S4
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    Image Search Results


    Spatial RNAseq further suggests boundary identity, cytokinin signaling, and brassinosteroid signaling are increased in drmy1 . A) Cartoon of a typical transverse section of an ap1cal 35S::AP1-GR cauliflower-like cluster, showing the developing flower buds with initiating sepals on the outer ring . B) Representative brightfield images of transverse sections of WT and drmy1 in the ap1cal 35S::AP1-GR background. n=9 Total WT replicate cauliflower-like clusters, n=22 drmy1 replicate cauliflower-like clusters (See also Figure S3). C) An overlay of which single cell cluster maps with the highest prediction score to each Visium spot on the same tissue shown in panel (B). See figure S3 for a more detailed view of prediction scores from each single cell cluster. D) The proportions of Visium spots grouped by which single cell cluster mapped to each with the highest prediction score (clusters that mapped to <10 spots in either genotype were omitted). For cluster 2 (boundaries), WT = 0.102, drmy1 = 0.232. E-G) Overlays of the percentage of transcripts from cytokinin downregulated genes (E), brassinosteroid downregulated genes (F), and SCT normalized expression of DWF1 , a brassinosteroid biosynthesis gene (G). H) Dotplot showing the differences in expression of genes up and down-regulated by various hormones. For each cell, the percentage of transcripts that come from the set of genes up or downregulated by applications of these hormones according to is calculated, then the mean of all the cells in each cluster is calculated for WT and drmy1 . The size of each dot represents the log of the inverse of the p-value between the mean of the two genotypes with a Bonferroni adjusted threshold of p < 0.00069. The color of each dot represents the percentage increase or decrease of the mean of drmy1 compared to WT. Scale bars 500 μm See also: Figure S3, S4

    Journal: bioRxiv

    Article Title: Brassinosteroids mediate proper coordination of sepal elongation

    doi: 10.1101/2025.07.13.664398

    Figure Lengend Snippet: Spatial RNAseq further suggests boundary identity, cytokinin signaling, and brassinosteroid signaling are increased in drmy1 . A) Cartoon of a typical transverse section of an ap1cal 35S::AP1-GR cauliflower-like cluster, showing the developing flower buds with initiating sepals on the outer ring . B) Representative brightfield images of transverse sections of WT and drmy1 in the ap1cal 35S::AP1-GR background. n=9 Total WT replicate cauliflower-like clusters, n=22 drmy1 replicate cauliflower-like clusters (See also Figure S3). C) An overlay of which single cell cluster maps with the highest prediction score to each Visium spot on the same tissue shown in panel (B). See figure S3 for a more detailed view of prediction scores from each single cell cluster. D) The proportions of Visium spots grouped by which single cell cluster mapped to each with the highest prediction score (clusters that mapped to <10 spots in either genotype were omitted). For cluster 2 (boundaries), WT = 0.102, drmy1 = 0.232. E-G) Overlays of the percentage of transcripts from cytokinin downregulated genes (E), brassinosteroid downregulated genes (F), and SCT normalized expression of DWF1 , a brassinosteroid biosynthesis gene (G). H) Dotplot showing the differences in expression of genes up and down-regulated by various hormones. For each cell, the percentage of transcripts that come from the set of genes up or downregulated by applications of these hormones according to is calculated, then the mean of all the cells in each cluster is calculated for WT and drmy1 . The size of each dot represents the log of the inverse of the p-value between the mean of the two genotypes with a Bonferroni adjusted threshold of p < 0.00069. The color of each dot represents the percentage increase or decrease of the mean of drmy1 compared to WT. Scale bars 500 μm See also: Figure S3, S4

    Article Snippet: To optimize RNA permeabilization, we used the Visium Spatial Tissue Optimization Slide & Reagent Kit, 4 slides (10x Genomics PN-1000193).

    Techniques: Expressing